W dniach 25-27 maja 2022 w Sofii w Bułgarii odbyła się Międzynarodowa Konferencja na temat Metabarcodingu DNA i Bioróżnorodności. Nasz zespół z Uniwersytetu Łódzkiego zaprezentował na niej poster opisujący projekt ECOPOND.
Miejsce: International Conference on DNA Barcoding and Biodiversity, 25-27.05.2022, Sofia, Bulgaria
Osoby: Baranowska M (prezenterka) / Baranowska M, Krawczyk D, Janik-Superson K, Królikowska K, Seweryn M, Lach J, Tończyk G, Desiderato A, Taugbøl A, Strand DA, Brodecki J, Strapagiel D, Bącela-Spychalska K.
Tytuł: How does urbanisation impact pond biodiversity in a geographical gradient?
Monika Baranowskaa, Dawid Krawczyka, Katarzyna Janik-Superson
Abstrakt:
Urbanization is a process inducing the loss of natural habitats and one of the reasons of harmful changes in ecosystems. Urbanization leads to “biotic homogenization” and opens a gate for invasive species. Ponds are freshwater habitats existing in both urban and non-urban landscapes and they
are biodiversity hotspots of many rare and endangered species. However, as these waterbodies are not monitored within a systematic manner, the knowledge about overall pond biodiversity still remains poor. The aim of the present study is to assess how urbanization impact invertebrate pond diversity in the context of a geographical gradient, spanning from the southern Poland to the idle of Norway, based on environmental DNA. Including metabarcoding of bacteria and fungi helped us
evaluate the ecological quality of studied waterbodies. Pond water was sampled and filtered from replicated urban and rural ponds in close geographic proximity, within five geographic regions
(Krakow – Trondheim). Characteristics of each pond was described on site and the water chemistry (N, P, TOC, pH, conductivity), microplastic content and distance from metropolia were measured to determine the level of anthropogenic pressure. Environmental DNA was isolated from filters with different pore sizes: 2.0, 0.45 and 0,22μm. The isolation was done with the Dneasy Power Soil Kit adding Proteinase K to the protocol. DNA libraries were prepared using PCR-based amplification of the different regions depending of organism identification (16S rRNA for bacteria, LSU rRNA for fungi, COI for invertebrates). Sequencing was performed in a paired-end modality on the Illumina MiSeq platform rendering 2 x 250 bp paired-end sequences. Alpha- and beta-diversity comparisons taking for account urban and rural environments and geographical range were done. The results will be shown during presentation.